Volume 3, Issue 3, September 2018, Page: 40-46
Expression and Role of Sphingosine 1-Phosphate Receptors in Intervertebral Disc Degeneration
Kuang Yang, Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, P. R. China
Kang Lu, Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, P. R. China
Wei Zhang, Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, P. R. China
Haiyin Li, Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, P. R. China
Changqing Li, Department of Orthopedics, Xinqiao Hospital, the Third Military Medical University, Chongqing, P. R. China
Received: Aug. 30, 2018;       Accepted: Sep. 13, 2018;       Published: Oct. 26, 2018
DOI: 10.11648/j.cbe.20180303.14      View  158      Downloads  4
Abstract
Background: e mechanism of intervertebral disc degeneration is unclear, inflammation may play an important role in it. Sphingosine 1-phosphate (S1P) is an important lipid mediator, has been confirmed to be implicated in many inflammation processes.Purpose to study the expression and role of S1PRs in the intervertebal disc (IVD) degeneration to enhance understanding of disc degeneration. Methods Degenerated and normal IVD were harvested from patients with IVD degeneration via surgery operation. The degenerated grade of each IVD specimen was assessed by Histological method. Expression of S1P receptor subtypes in each specimen was evaluated using real-time PCR, immunohistochemistry, and western blotting. The effect of S1PR on inflammation induced by interleukin-1β in nucleus pulposus (NP) cells was also assessed by real time PCR and western blot. Results The nucleus pulposus mainly expressed theS1PR1/2/3, and the expression decreased in the severe degenerated nucleus pulposus cells. The ligand, S1P, inhibited the up-regulation of matrix metallopeptidase-3(MMP-3) and ADAM metallopeptidase with thrombospondin type 1 motif 4(ADAMTS4) induced by IL-1β. Conclusions The results show that an the expression of S1PRs in degenerative discs is down-regulated as degeneration, and S1P can inhibit the inflammation response induced by IL-1β in NP cells, implicating that S1P/S1PR may contribute to IVD degeneration.
Keywords
Phingosine 1-Phosphate Receptors, Nucleus Pulposus Cells, Intervertebral Disc Degeneration, IL-1β
To cite this article
Kuang Yang, Kang Lu, Wei Zhang, Haiyin Li, Changqing Li, Expression and Role of Sphingosine 1-Phosphate Receptors in Intervertebral Disc Degeneration, Chemical and Biomolecular Engineering. Vol. 3, No. 3, 2018, pp. 40-46. doi: 10.11648/j.cbe.20180303.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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